Journal: Frontiers in Oncology

Article Title: The role of tumor-associated macrophages in glioma cohort: through both traditional RNA sequencing and single cell RNA sequencing

doi: 10.3389/fonc.2023.1249448

Figure Lengend Snippet: (A) The cck8 assay in U87 cells; (B) The cck8 assay in U251 cells; (C) The Edu assay in U87 cells; (D) The Edu assay in U251 cells.

Article Snippet: Human glioma cell lines U251 and U87 were purchased from Beijing Zhongyuan Heju Biotechnology Co., Ltd. (Beijing, China).

Techniques: CCK-8 Assay, EdU Assay

Journal: International Journal of Molecular Sciences

Article Title: LPPR5 Expression in Glioma Affects Growth, Vascular Architecture, and Sunitinib Resistance

doi: 10.3390/ijms23063108

Figure Lengend Snippet: In-silico analysis of LPPR5 in physiological brain tissue and primary brain tumor. ( a ) LPPR5 expression is physiologically high in human adult brain. Outside the CNS, LPPR5 is compara-tively highly expressed in testis [GTeX gene expression data for LPPR5 in brain (blue, Hippocampus light green) and non-brain tissue (dark green). Expression values are shown in TPM (Tran-scripts Per Million), calculated from a gene model with isoforms collapsed to a single gene. No other normalization steps have been applied. Box plots are shown as median and 25th and 75th per-centiles; points are displayed as outliers if they are above or below 1.5 times the interquartile range]. ( b ) LPPR5 is significantly un-der-expressed in (high-grade) glioma. Relative under-expression rank is median rank across all analyses in gene expression in Sun Brain, and respectively Roth normal in Oncomine [p for medi-an-ranked analysis, Oncomine visualization and statistical anal-ysis]. ( c ) A subgroup of high-grade glioma has decreased LPPR5 expression; in lower grade glioma, the ratio of tumors with low LPPR5 expression is smaller [mRNA expression data from Sun Brain, each bar represents a single tumor, II and III indicates astrocytoma WHO grade]. ( d ) On the DNA-level, LPPR5 is deleted in most oligodendrogliomas and to a lesser degree in lower grade astrocytic gliomas and GBM [Oncomine visualization of LPPR5 DNA copy number units of the tumors in Kotliarov Brain, each bar represents a single tumor]. ( e ) LPPR5 expression is significantly lower in the mesenchymal expression subtype of GBM ( n = 49) compared to classical ( n = 39, * p = 0.0451) and proneural ( n = 29, **** p < 0.0001) subtypes. Expression in the neural subtype ( n = 26) is significantly lower than in proneural subtype (* p = 0.0162) [TCGA via cBio]. ( f ) Histogram plot of 70 human Glioma cell lines screened, 50 show low expression of LPPR5 [Cancer Cell Line Encyclopedia, Broad Institute].

Article Snippet: Moreover, in a screening of 70 human glioma cell lines from the Cancer Cell Line Encyclopedia (Broad Institute), only 4 cell lines showed a significant expression of LPPR5 (LN319, KNS42, TM31, and NMCG1; f).

Techniques: In Silico, Expressing, Gene Expression